人 EAR1 (Eosinophil Associated

RiboQuant™ Ribonuclease Protection Assay System

相关专题 核糖核酸酶保护实验 对于放射性RPA，杂交双链进行变性聚丙酰胺凝胶电泳 ，用放射自显影或磷屏成像系统检测被保护的探针的信号。 对于非放的RPA，杂交双链经过变性聚丙酰胺凝胶电泳 后电转移至尼龙膜，UV照射使被保护的RNA探针与膜交联而固定，再用试剂盒提供的链霉亲和素-辣根过氧化物酶(Streptavidin-HRP)和化学发光底物与膜上biotin标记的探针结合，这样，再将膜放入暗盒中暴露于X射线

Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells

The pregnancy-associated glycoproteins (PAGs) are a large gene family expressed in trophoblast cells of ruminant ungulates. The detection of PAGs in maternal serum has served as the basis for pregnancy detection in ruminant ungulates

ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases

. The cytochrome c ELISA is generally used to quantify the activities of the proteins belonging to the Bcl-2 family. The active caspase ELISA quantifies a specific active caspase among a background of latent caspase and other active caspases.