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文献和实验Microarray Analysis of DNA Replication Timing
here for generation of high-resolution temporal maps of DNA replication in synchronized human cells. This method does not require amplification of DNA before microarray hybridization and so avoids errors introduced during PCR. A major advantage of using this procedure
In Vitro Immunogenicity Risk Assessment of Therapeutic Proteins in Preclinical Setting
-alpha (rHuEPO)and recombinant Factor VIII (rFVIII). Flow-cytometry was used to determine the maturation level of dendritic cells (DCs), a primary initiator of T-cell responses. Studies to capture T-lymphocyte proliferation upon challenge with free rFVIII
supporting material, namely, polymer coated initiator integrated poly(dimethysiloxane) (iPDMS), which was able to achieve such “absolute” zero (i.e., below the detection limit of instrument). The implementation of this iPDMS enables practical and high-quality
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