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文献和实验Numerous studies on telomerase expression have consistently demonstrated the presence of telomerase activity in the vast majority of different types of cancer, as well as immortalized cells, but have failed to detect telomerase in most normal
PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
for the parallel analysis of the activities of more than 200 transcription factor proteins, using an advanced oligonucleotide array-based transcription factor assay (OATFA) platform, to assay TF activities in mice. The system uses a PCR-based system to translate
时样本之间干扰。 Q:说明书上写细胞量为 1~5 × 105 ,细胞量多的话对检测结果有没有影响? A:建议细胞量最多不要超过 106个,过量的细胞相当于染料被稀释,影响染色和分离效果。一般 2×105 个细胞足够进行凋亡检测了,通常情况下检测 10,000 个细胞就能得到比较好的结果。 Q:是否可以用 PBS 替代凋亡试剂盒里面的 Binding Buffer? A:不行,Binding Buffer 是必须要加的,Annexin V 与 PS 的结合依赖 Ca2+,Binding
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