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文献和实验Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA
Major histocompatibility class I and II (MHC-I and MHC-II) antigens (Ags), as well as a variety of cell surface receptors, have been shown to enter the endocytic pathway of antigen-presenting cells (APCs). MHC Ags endocytose into clathrin
thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
β随着其他因子的影响,其表达水平也会发生相应的变化,一般客户很难察觉,因此这样的恶性造假行为使得消费者被动造假,后果极其严重,因此善意提醒客户警惕此类作假试剂盒!鉴别方法:Ⅰ. 利用干扰实验即可辨别ELISA试剂盒是否检测目的抗原,方法如下:(1)将针对试剂盒特异性的重组蛋白抗原和试剂盒中的检测抗体配置成antibody: antigen≈1:2的混合孵育液。(2)37℃孵育15分钟后,代替原试剂盒中的检测抗体。(3)后续操作按照试剂盒说明书进行,加检测抗体、酶复合物和底物。(4)实验完毕后,检测其标准
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