人 PCI (Procollagen I) ELISA试剂盒

Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide

on a polyclonal antibody raised against recombinant human exon 2 fusion protein of type II procollagen. We show that this ELISA is highly specific for circulating PIIANP and has adequate technical precision. In patients with knee osteoarthritis and rheumatoid

Production of Monoclonal Antibody by DNA Immunization with Electroporation

against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA

获得高质量土壤RNA的10个建议

的PCI非常重要，在试剂盒操作说明书中我们给出了一些使用建议。PCI比例必须为25：24:1，pH介于6.7-8，并保存在pH8.0的TE缓冲液中。许多人提取动物组织和细胞习惯性地只使用地pH值的苯酚来提取RNA。而对于土壤，pH中性的苯酚会合适。 3. 优化异丙醇浓缩（步骤12） PCI裂解步骤后加入SR3溶液，下一步加入异丙醇沉淀总核酸。如果你在做底泥，加完SR3溶液后总体积可能超过5ml。增加SR4（异丙醇）的使用量，至与步骤11获得的样品体积相同，以保证充分沉淀核酸。