- ￥673 - 3699
- DIMA BIOTECH/缔码生物
- 武汉
- PME100322
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant human KRAS protein with C-terminal mouse Fc tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥673.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2466.0 |
| 规格： | 100μg | 产品价格： | ￥3699.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | KRAS |
| Uniprot ID | P01116 |
| 别名 | C-K-RAS;c-Ki-ras2;CFC2;K-Ras;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KI-RAS;KRAS1;KRAS2;NS;NS3;RALD;RASK2 |
| 背景 | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| 产品英文名称 | Recombinant human KRAS protein with C-terminal mouse Fc tag |
| 分子结构 | KRAS(Thr2-Cys185) mFc(Pro99-Lys330) |
| 分子量 | The protein has a predicted molecular mass of 47.1 kDa after removal of the signal peptide. The apparent molecular mass of KRAS-mFc is approximately 35-55 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | C-mouse Fc Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human KRAS Protein, mFc Tag on SDS-PAGE under reducing condition.
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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
Bait Protein Multivalent Complexes on Protein A Microbeads Human IgG (Jackson ImmunoResearch, cat. no. 009‐000‐003
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
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