- ￥818 - 4499
- DIMA BIOTECH/缔码生物
- 武汉
- PME100569
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant Human M-CSF with C-terminal 6×His tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥818.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2999.0 |
| 规格： | 100μg | 产品价格： | ￥4499.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | M-CSF |
| Uniprot ID | P09603 |
| 别名 | CSF-1;MCSF |
| 背景 | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| 产品英文名称 | Recombinant Human M-CSF with C-terminal 6×His tag |
| 分子结构 | M-CSF(Glu33-Arg255) 6×His tag |
| 分子量 | The protein has a predicted molecular mass of 26 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-His is approximately 25-55 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | C-6×His Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human M-CSF Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human M-CSF Protein, His Tag ( PME100569) can bind Anti-M-CSF Neutralizing antibody BME100104 in a linear range of 2.44–156.25 ng/mL.
Figure 3. Flow cytometry analysis with 1 μg/mL Human M-CSF Protein, His tag (PME100569) on HEK293 cells transfected with human CSF1R (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
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文献和实验.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
photoprotein aequorin by immobilized metal-ion affinity chromatography, Protein Express. Purif. 27 (2003) 384-390. 33. Y. Shi, C. Jiang, Q. Chen, H. Tang, One-step on-column affinity refolding purification and functional analysis of recombinant human VDAC
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