- ￥727 - 3999
- DIMA BIOTECH/缔码生物
- 武汉
- PME100531
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant Human SIRPa with C-terminal mouse Fc tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥727.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2666.0 |
| 规格： | 100μg | 产品价格： | ￥3999.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | SIRPa |
| Uniprot ID | P78324 |
| 别名 | SHPS1;SIRPA;CD172A;BIT;MFR;MYD1;P84;PTPNS |
| 背景 | Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. |
| 产品英文名称 | Recombinant Human SIRPa with C-terminal mouse Fc tag |
| 分子结构 | SIRPa(Glu31-Tyr373) mFc(Pro99-Lys330) |
| 分子量 | The protein has a predicted molecular mass of 63.8 kDa after removal of the signal peptide.The apparent molecular mass of SIRPa-mFc is approximately 70-100 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | C-Mouse Fc Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human SIRPα Protein, mFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human SIRPα Protein, mFc Tag (PME100531) can bind anti-SIRPα antibody, Rabbit mAb clone: DM8 in a linear range of 3.2-80 ng/mL.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human SIRPα Protein, mFc Tag (PME100531) can bind Human CD47 full length protein-synthetic nanodisc (FLP100039) in a linear range of 80–2000 ng/mL.
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文献和实验.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
photoprotein aequorin by immobilized metal-ion affinity chromatography, Protein Express. Purif. 27 (2003) 384-390. 33. Y. Shi, C. Jiang, Q. Chen, H. Tang, One-step on-column affinity refolding purification and functional analysis of recombinant human VDAC
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