人CD27重组蛋白/Human CD27 Protein,

产品类型	ECD Proteins
靶点	CD27
Uniprot ID	P26842
别名	CD27;TNFRSF7;S152;T14;Tp55
背景	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
产品英文名称	Recombinant human CD27 protein with C-terminal mouse Fc and 6×His tag
分子结构	CD27(Ala20-Arg191) mFc(Pro99-Lys330) 6×His
分子量	The protein has a predicted molecular mass of 65-72 kDa after removal of the signal peptide.
纯度	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
标签	C-Mouse Fc and 6×His Tag
有无偶联	Unconjugated
表达宿主	HEK293
组分	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
货期	In Stock
储存与运输	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
应用范围	Research use only

Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein (PME100013) can bind Anti-CD27 Neutralizing antibody BME100018 in a linear range of 0.64-16.0 ng/ml.

Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein  (PME100013) can bind Human CD70,hFc-His tagged protein PME100486 in a linear range of 3.2-80 ng/ml.

Figure 4. Flow cytometry analysis with 15 μg/mL Human CD27 Protein, mFc-His tag (PME100013) on HEK293 cells transfected with human CD70 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 5. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD27 Protein, mFc-His Tag (PME100013) can bind Anti-CD27(boserolimab biosimilar) mAb (BME100439) in a linear range of 1.6–8 ng/mL.