- ￥2450
- AntibodySystem
- France
- RHE14403
- 2025年07月05日
- IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
- 克隆性：
Monoclonal
- 适应物种：
Human, Mouse, Rat
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
IF, IHC, WB
- 浓度：
1 mg/ml
- 靶点：
C/EBP-homologous protein, DDIT3, DDIT-3, CCAAT/enhancer-binding protein homologous protein, C/EBP zeta, CHOP-10, Growth arrest and DNA damage-inducible protein GADD153, DNA damage-inducible transcript 3 protein
- 抗体英文名：
Anti-CHOP/GADD153/DDIT3 Antibody (R3J90)
- 抗体名：
Anti-CHOP/GADD153/DDIT3 Antibody (R3J90)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human, Mouse, Rat |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | IF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Applications | IF, IHC, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | C/EBP-homologous protein, DDIT3, DDIT-3, CCAAT/enhancer-binding protein homologous protein, C/EBP zeta, CHOP-10, Growth arrest and DNA damage-inducible protein GADD153, DNA damage-inducible transcript 3 protein, GADD153, CHOP10, C/EBP-homologous protein 10, CHOP |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P35638 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3J90 |
| Note | For research use only. |
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文献和实验Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Protocol for anti-HA antibody Western Blotting
room. Expose film for ~2 mins. Notes on the primary antibody: Source - Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in ―20c
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
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