Single Cell WGA Kit DOP-PCR法

CellTrace CFSE Cell Proliferation Kit

Materials Needed but Not Included: Cells of interest as a single-cell suspension PBS/0.1% BSA 5 mM stock solution of CFSE probe (see Reagent Preparation) Culture media for cells of interest Flow cytometer with 488

PureLink RNA Mini Kit

                        ii.            Add 0.3 or 0.6 mL Lysis Buffer with 2-mercaptoethanol to the sample (see table above for volume).                       iii.            Vortex until the cell pellet is dispersed and the cells appear lysed

Dynabeads® mRNA DIRECT Kit

of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes