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- 2025年07月12日
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文献和实验Biotin labeling and hybridization of probe to chromosomes
times more than the probe DNA. Hybridization of probe to chromosomes. Denature the mixture by placing at 75 C for 5 min, immediately chilling on ice for 5 min, and spining down the solution. Place the slides (by using
DNA Probe Purification Protocol
microgram DNA due to the limiting nucleotide concentrations used. In this case, a carrier such as yeast tRNA should be added to Buffer DP to a final concentration of 2-5 ug/mL before use to ensure binding to HiBind resin. The mixture is stable at -70℃
2-Aminopurine as a Probe for Quadruplex Loop Structures
, there is a rich library of theoretical and practical materials available to aid in data interpretation. The intrinsic fluorescence of most nucleic acids is too low to be useful in structural studies. Thus, it is necessary to incorporate a suitable
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