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文献和实验Isolation of cell nuclei for the application in the cell-free system
Leupeptin 100 µl of 1 mg/ml Pepstatin 100 µl of 1 mg/ml Aprotinin 37.65 ml H2O nuclease free NUCLEI STORAGE BUFFER (NSB): COMPOSITION: RECIPE for 1 ml: 10 mM PIPES (pH 7.4) 5 mM EGTA 80 mM KCl 20 mM NaCl 50% Glycerol 250 mM Sucrose
Preparation of cytoplasmic extracts for the application in a cell-free system
EGTA 250 µl of 1 M KCl 100 µl of 100 mM MgCl 5 µl of 1 M DTT 50 µl of 10 mM PMSF 10 µl of 1 mg/ml Leupeptin 10 µl of 1 mg/ml Pepstatin 10 µl of 1 mg/ml Aprotinin 2.04 ml H2O nuclease free
The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos
Objective The purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and at the initiation of gastrulation
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