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2mL
产品货号:XF-J5896
产品规格:2mL
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文献和实验RNA Analysis by Nuclease Protection
2A ) 10× transcription buffer (see recipe ) 3NTP mix (see recipe
For hybridization, make pre-mix of salts in a separate tube (will use 4 µl of pre mix/rxn): Pre-mix: 2 µl 5M NaCl 1.6 µl 0.5M PIPES, pH 7.0 0.2 µl 0.1M EDTA, pH 7.0 0.2 µl water 3. Add the following to your sample: 16 µl formamide 4 µl pre-mix salts
Nested Deletions Using Exonuclease-III and Mung Bean Nuclease
7.5U mung bean nuclease (diluted in mung bean dilution buffer, see end) per 2.5ug time point and incubate at 30° for 30 min. Ethanol ppte, resuspend and load onto a 0.8% TAE gel. Run and excise bands free from undigested DNA. Isolate DNA
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