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文献和实验TRICINE GELS Protocol (For Low MW proteins (
Stock Solutions1) Gel Buffer: 3 M TrisHCl pH8.45 + 0.3% SDS. Keep RT. (Prepare: 18.15gr Tris + 150mg SDS + H2O up to 50ml. Adjust pH before you add the SDS) 2) 40% Acrylamide solution 29:1 (Acrylamide 29 / Methylene bis Acrylamide 1) or 40
Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers
to the more mobile Tricine (N-tris[hydroxymethyl]-methylglycine) and by lowering the pH of the separating gel.
2O. Degas under vacuum 10 to 15 minutes. Add 50 µl of 10% ammonium persulfate and 10 µl TEMED. Swirl gently to mix. Use immediately. Produces 10 ml of stacking gel, sufficient for four minigels. Separating gel (16.5% T 6.0% C) In a 50-ml
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