Precast-Gel 变性预制胶 4-15%,15 wel

RNA甲醛变性胶电泳

即水饱和的溴酚蓝液。在15ml灭菌离心管中，依次加入以下各种成分：4.0ml 10 × FA buffer3.1ml 甲酰胺2.0ml 100%的甘油720ul 37%的甲醛80ul0.5M的 EDTA （PH 8.0）16ul 水饱和的溴酚蓝100ul DEPC水混匀，分装，-20℃保存，常用放4℃保存。5、1×甲醛变性胶电泳缓冲液（1×running buffer）：20 ml 10×FA gel buffer，4.0 ml 37%的甲醛，176 ml水，放入电泳槽中使用，一般在3次电泳结束后需

Agarose Gel Electrophoresis

in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes

SDS Gel Electrophoresis of Tubulin\MAPs

% TCA Procedure   Prepare 15 cm glass tubes with 6 mm internal diameter. Polymerize a 10 cm separation gel and a 1 cm stacking gel within the tubes by the addition of TEMED and ammonium persulfate as directed in Chapter Four.