Precast-Gel 变性预制胶 6%,10 wells,

RNA甲醛变性胶电泳

即水饱和的溴酚蓝液。在15ml灭菌离心管中，依次加入以下各种成分：4.0ml 10 × FA buffer3.1ml 甲酰胺2.0ml 100%的甘油720ul 37%的甲醛80ul0.5M的 EDTA （PH 8.0）16ul 水饱和的溴酚蓝100ul DEPC水混匀，分装，-20℃保存，常用放4℃保存。5、1×甲醛变性胶电泳缓冲液（1×running buffer）：20 ml 10×FA gel buffer，4.0 ml 37%的甲醛，176 ml水，放入电泳槽中使用，一般在3次电泳结束后需

Agarose Gel Electrophoresis

in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes

2D Gel Electrophoresis（图）

of distance migrated vs. log10MW for known samples, and read off the logMr of the sample after measuring distance migrated on the same gel. Sample Preparation. Sample preparation is a key factor in successful 2DE, with complete solubilisation