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- XF-J5192
- 2025年07月11日
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- 规格：
500mL
产品货号：XF-J5192
产品规格：500mL
产品包装：袋
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文献和实验Preparing and Running a Protein Gel (7% Polyacrylamide)
plate faces the apparatus and the foam blocks form a seal with the gasket, and clamp it onto the gel apparatus using spring clips on the sides. 4) Pour 1x Running Buffer into the upper buffer chamber of the gel apparatus and check for leaks
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
8.3 with acetic acid at room temp.Adjust volume to 500 ml.Running buffer is 1X TGOE buffer[Note that there is no Mg or EDTA in both the gel and in the running buffer.]
SDS-PAGE Western Blotting Protocols
is the same as the that of the spacers’. 6. Remove comb. 7. Pour resolving gel to the mark in step 5. Optional: Create an agarose plug with newly liquified 1% agarose (i.e., the agarose needs to be hot when used). The agarose does not affect the running of the SDS-PAGE
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