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- 2025年07月13日
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文献和实验Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
Plant DNA methylation analysis by bisulfite genomic sequencing
) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml
the probe stay for 1.5 hrs RT for complete erythrocyte lysis Count platelets under a phase contrast microscope at x400 magnification using Neubauer hemocytometer SOLUTIONS The Unopette Reservoir
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