磷脂酶A2，200u/ml

TIL （肿瘤浸润淋巴细胞）细胞的培养

） ● 培养液：RPMI1640 培养液＋10％AB 型血清＋IL－2（200u/ml）或X－VIVO15 培养液 ● 消化液：IV 型胶原酶（230u/g）、I 型DNA 酶3600u 、V 型透明质酸酶（1500u/g） ● 器皿与仪器：75cm2 培养瓶，15ml 聚丙烯试管，手术刀、剪、镊，CO 2 孵箱，三角烧瓶，磁力搅拌器等 实验操作 1.在无菌条件下将切除新鲜瘤体组织去除坏死部分与结缔组织，用RPMI1640 培养液冲洗干净

Protein Expression and Purification of MBP-T antigen (131-259)

into 150 ml medium.For 6 L labeling prep.set up 2 x 150 ml of cultures into M9 labeling medium) iv.Transfer 25 ml culture into 1 L LB medium (or 50 ml of M9 culture into 1 L M9-labeling medium)containing Kan.Grow cells at 37 ℃ until the cells reach an OD

viral immunity

the probe stay for 1.5 hrs RT for complete erythrocyte lysis Count platelets under a phase contrast microscope at x400 magnification using Neubauer hemocytometer SOLUTIONS The Unopette Reservoir