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文献和实验The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos
Objective The purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and at the initiation of gastrulation
Measurement of Intracellular Calcium with Fluorescent Calcium Indicators
Changes in cytosolic free calcium ion concentration ([Ca2+ ]i ) accompany many cellular transitions and stimulation of cell surface receptors. Both release of Ca2+ from internal stores and Ca2+ entry through plasma membrane ion channels
The involvement of calcium (Ca) in living organisms has long been recognized, particularly its presence in invertebrate shells and vertebrate bones. It was not, however, before the studies of S. Ringer (1882), who showed that its presence
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