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文献和实验Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis
Killer toxin activity is commonly determined by the well test (1 ), which ensures the inhibition zone on a substratum of sensitive strain cells. The Rhodamine B assay described in Chapter 31 is based on estimation of the fraction of killed
Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes
Killer yeasts secrete proteins (killer toxins, zymocins) that kill sensitive yeast strains (1 ,2 ). The killer strain is immune (R+ ) to the effects or its own toxin, but it can be sensitive to the toxins of another immunity group
Rhodamine-Phalloidin/Calcofluor Staining
Rhodamine-Phalloidin/Calcofluor Staining David Amberg Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K for 5 min
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