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1g
产品货号:XF-J2725
产品规格:1g
产品包装:瓶
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文献和实验Measurement of Oxidized/Reduced Glutathione Ratio
, acting as a free radical scavenger and inhibitor of lipid peroxidation. GSH also participates in the detoxification of hydrogen peroxide by various glutathione peroxidases. The ratio of reduced GSH to oxidized GSH (GSSG) is an indicator of cellular health
GSH和GSSG 参照 Anderson等 (1992)。取0.5 g样品,加入3 mL冰冷的6%的偏磷酸(含1 mmol•L-1 EDTA , pH 2.8),冰浴研磨,匀浆液以20,000 g,4 ℃离心15 min,取上清液来马上测定GSH和GSSG的含量或储存在-20 ℃下等待测定。总的GSH和GSSG含量测定如下:200 μL提取液加 1.2 mL反应液包含400 μL反应液1(110 mmol•L-1 Na2HPO4•7H20, 40 mmol•L-1 NaH2PO4•H2
Reduced Glutathione and Glutathione Disulfide
Glutathione (GSH; L- -glutamyl-L-cysteinl-glycine) plays an important role in the prevention of radical mediated injury to the body. It does so as a radical scavenger and by supplying GSH to the antioxidant enzymes described in Chapter 29
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