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产品货号:XF-J2610
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文献和实验Clinical Trials With GDEPT: Cytosine Deaminase and 5-Fluorocytosine
. Several combinations have been described for GDEPT, one of these being the cytosine deaminase/5-fluorocytosine system. Cytosine deaminase (CD) is a nonmammalian enzyme that catalyses the hydrolytic deamination of cytosine to uracil. Therefore, it is capable
药物靶向治疗(drugs targeting)机理可概括为病毒导向酶的药物前体治疗(virus directed enzyeme prodrug therapy,VDEPT),即用反转录病毒载体的外源基因转移到细胞内.该基因编码一种酶,此酶可将一种无害的药物前体转变为细胞毒素复合物。带有这一基因的病毒载体只在特殊组织或肿瘤细胞中而不在正常细胞中表达。例如,胞嘧啶脱氨酶(cytosine deaminase)可将无害的5-氟胞嘧啶(5-fluorocytosine,5-FC)转变为细胞
Transfection of Rodent Malaria Parasites
a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals
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