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- 详细信息
- 文献和实验
- 技术资料
- 规格:
1mL*10
产品货号:XF-J0446
产品规格:1mL*10
产品包装:瓶
储存条件及有效期:详见标签
本产品仅供科研实验用,不做其他用途!
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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time: 2 x 1 ml CHIP lysis buffer 2 x 1 ml CHIP
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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