- ￥2450
- AntibodySystem
- France
- RHJ81201
- 2025年08月13日
- ELISA, FCM, IF
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
ELISA, FCM, IF
- 浓度：
1 mg/ml
- 靶点：
ALK, ALK tyrosine kinase receptor, CD246, Anaplastic lymphoma kinase
- 抗体英文名：
Anti-CD246/ALK Antibody (R3X50)
- 抗体名：
Anti-CD246/ALK Antibody (R3X50)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:250 |
| Applications | ELISA, FCM, IF |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | ALK, ALK tyrosine kinase receptor, CD246, Anaplastic lymphoma kinase |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q9UM73 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3X50 |
| Note | For research use only. |
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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