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- AntibodySystem
- France
- RHJ69702
- 2025年07月12日
- IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
IHC
- 浓度：
1 mg/ml
- 靶点：
Interleukin-1 homolog 1, IL1H1, IL-1H1, IL-1RP2, IL-1 epsilon, IL1E, IL-1F9, IL36G, IL-1-related protein 2, Interleukin-1 family member 9, IL1RP2, Interleukin-36 gamma, Interleukin-1 epsilon, IL1F9
- 抗体英文名：
Anti-IL36G/IL-1F9 Antibody (R3X05)
- 抗体名：
Anti-IL36G/IL-1F9 Antibody (R3X05)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG2a |
| Conjugation | |
| Tested applications | IHC: 1:50-1:200 |
| Applications | IHC |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | Interleukin-1 homolog 1, IL1H1, IL-1H1, IL-1RP2, IL-1 epsilon, IL1E, IL-1F9, IL36G, IL-1-related protein 2, Interleukin-1 family member 9, IL1RP2, Interleukin-36 gamma, Interleukin-1 epsilon, IL1F9 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q9NZH8 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3X05 |
| Note | For research use only. |
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Centrifuge cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition
ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
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