- ￥2450
- AntibodySystem
- France
- RHD46603
- 2025年07月13日
- ELISA, IHC, WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
ELISA, IHC, WB
- 浓度：
1 mg/ml
- 靶点：
CD73, 5'-nucleotidase, NT5E, NT5, 5'-NT, Ecto-5'-nucleotidase, NTE
- 抗体英文名：
Anti-CD73/NT5E Antibody (R3F89)
- 抗体名：
Anti-CD73/NT5E Antibody (R3F89)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | ELISA: 1:10000, IHC: 1:200-1:1000, WB: 1:500-1:2000 |
| Applications | ELISA, IHC, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | CD73, 5'-nucleotidase, NT5E, NT5, 5'-NT, Ecto-5'-nucleotidase, NTE |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P21589 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3F89 |
| Note | For research use only. |
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文献和实验Single Primer ("Semi-Random") P
cresol red 2. T/TS 10.5 µL EDB 1 µL Taq Polymerase (Promega) @ 5 u/µL 1 µL TaqStart antibody (CloneTech) as delivered 3. EDB 2.5 mg/ml bovine serum albumin in 10 mM Tris, pH 8.3 References 1. S.R.J.A.M. Hermann
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
ChIP‐Seq: A Method for Global Identification of Regulatory Elements in the Genome
. The process is initiated by cross?linking DNA and DNA?bound proteins. Subsequently, chromatin is isolated from nuclei and subjected to sonication. An antibody against a specific TF or DNA?binding protein is then used to immunoprecipitate specific DNA?TF
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