- ￥2450
- AntibodySystem
- France
- RHD15013
- 2025年07月12日
- IF, WB
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
- 克隆性：
Monoclonal
- 适应物种：
Human, Mouse
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
IF, WB
- 浓度：
1 mg/ml
- 靶点：
H2a/x, Histone H2A.X, H2AFX, Histone H2AX, H2AX
- 抗体英文名：
Anti-Phospho-Histone H2A.X (Ser139) Antibody (R3E27)
- 抗体名：
Anti-Phospho-Histone H2A.X (Ser139) Antibody (R3E27)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human, Mouse |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | IF: 1:50-1:200, WB: 1:500-1:1000 |
| Applications | IF, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | H2a/x, Histone H2A.X, H2AFX, Histone H2AX, H2AX |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P16104 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3E27 |
| Note | For research use only. |
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文献和实验from the block they synchronously progress through S, G2 , and M. We recently reported that exposure of cells to these inhibitors at concentrations commonly used to synchronize cell populations led to phosphorylation of histone H2AX on Ser 139 (induction of γH2
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
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