- ￥2450
- AntibodySystem
- France
- RHC08102
- 2025年07月12日
- ELISA, FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
ELISA, FCM
- 浓度：
1 mg/ml
- 靶点：
T-cell surface glycoprotein CD3 delta chain, CD3D, T-cell receptor T3 delta chain, T3D, CD3d
- 抗体英文名：
Anti-CD3D Antibody (R2Y68)
- 抗体名：
Anti-CD3D Antibody (R2Y68)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | ELISA: 1:10000, FCM: 1:200-1:400 |
| Applications | ELISA, FCM |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | T-cell surface glycoprotein CD3 delta chain, CD3D, T-cell receptor T3 delta chain, T3D, CD3d |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P04234 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R2Y68 |
| Note | For research use only. |
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文献和实验Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection
Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature
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