- ￥2450
- AntibodySystem
- France
- RHB94408
- 2025年07月13日
- IHC, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
- 克隆性：
Monoclonal
- 适应物种：
Human, Mouse, Rat
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
IHC, WB
- 浓度：
1 mg/ml
- 靶点：
TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
- 抗体英文名：
Anti-TNFa/TNF-alpha Antibody (R2X41)
- 抗体名：
Anti-TNFa/TNF-alpha Antibody (R2X41)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human, Mouse, Rat |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Applications | IHC, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01375 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R2X41 |
| Note | For research use only. |
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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