Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱
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Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图

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  • ¥850 - 5500
  • 冠导生物
  • Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱
  • 美国、德国、欧洲等
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

      详见细胞说明资料

    • 细胞类型

      详见细胞说明资料

    • 肿瘤类型

      详见细胞说明资料

    • 供应商

      上海冠导生物工程有限公司

    • 库存

      ≥100瓶

    • 生长状态

      详见细胞说明资料

    • 年限

      详见细胞说明资料

    • 运输方式

      常温运输【复苏细胞】或干冰运输【冻存细胞】

    • 器官来源

      详见细胞说明资料

    • 是否是肿瘤细胞

      详见细胞说明资料

    • 细胞形态

      详见细胞说明资料

    • 免疫类型

      详见细胞说明资料

    • 物种来源

      详见细胞说明资料

    • 相关疾病

      详见细胞说明资料

    • 组织来源

      详见细胞说明资料

    • 英文名

      Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱

    • 规格

      1*10(6)Cellls/瓶

    "Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱
    BJ Cells人皮肤成纤维细胞保种中心|STR图谱
    SW948(DM) Cells人结肠腺癌细胞保种中心|STR图谱
    Beta-TC-6 Cells小鼠胰岛素瘤胰岛β细胞保种中心|STR图谱
    CMT-U27 Cells犬乳腺癌细胞保种中心|STR图谱
    GP293 Cells人胚肾细胞保种中心|STR图谱
    HepG2.2.15-LUC Cells人肝细胞保种中心|STR图谱
    CTLL-2 Cells小鼠T细胞保种中心|STR图谱
    PLC/PRF/5 Cells人肝癌细胞保种中心|STR图谱
    物种来源:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱来源于人源、鼠源等其它物种来源
    生长特性:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱贴壁生长
    公司细胞系培养稳定、耐活、复苏好,提供人源、鼠源、兔源、猪源等不同组织如肺、气管、支气管、甲状腺、胰腺、垂体、肾上腺、扁桃体、胸腺、肾、膀胱、输尿管、前列腺、心脏、血管等细胞系。全程提供细胞系组织来源、生长特性、细胞形态、背景资料、培养条件、冻存条件、参考文献、运输方式等产品信息及技术服务。
    细胞经过多次传代后,建立了稳定的体外培养模型且可稳定增殖。这些微小的生命单元在培养瓶中生生不息,恰似一支训练有素的军团,为科学家们科研实验提供着源源不断的创新灵感与技术支持。其价值不仅体现在当前广泛的应用中,更在于它为未来生命科学研究提供的可能性。随着技术的进步和对这一细胞系认识的深入,必将在更多领域展现其独特优势。研究人员在使用时应当充分了解其特性,合理设计实验,并注意细胞培养的质量控制,以确保研究结果的可靠性和可重复性。
    形态特性:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱上皮细胞样
    换液周期:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱每周2-3次
    产品包装:复苏发货:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)
    在细胞培养的日常操作中,细胞消化是一个看似简单却极易被误解的关键步骤。许多研究者,尤其是初学者,往往陷入过度消化的误区,认为必须将细胞吹打成完全离散的单细胞悬液才算消化完成。然而,这种认知不仅缺乏科学依据,更可能对细胞造成不可逆的损伤。事实上,绝大部分贴壁细胞的消化只需采用""胰酶润洗法""即可高效完成,即用胰酶润洗一遍后吸去,残留的微量胰酶在37℃条件下通常能在1-2分钟内使细胞充分脱离。这种方法的精妙之处在于利用难以精确计量的残留胰酶实现温和消化,既避免了酶解过度,又维持了细胞活性。
    ATCC细胞库(American Type Culture Colection),该中心一直致力于细胞分类、鉴定和保藏工作。ATCC是全球最大的生物资源保藏中心,ATCC通过行业标准产品、服务和创新解决方案支持全球学术、政府、生物技术、制药、食品、农业和工业领域的科学进步。ATCC提供的服务和定制解决方案包括细胞和微生物培养、鉴定、生物衍生物的开发和生产、性能测试和生物资源保藏服务。美国国家标准协会(ANSI)认可了ATCC标准开发组织,并制定了标准协议,以确保生物材料的可靠性和可重复性。ATCC的使命是为了获取、鉴定、保存、开发、标准化和分发生物资源和生物信息,以提高和应用生物科学知识。
    背景信息:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱细胞株分离自一个原发性结肠癌。当细胞长满时,表现出典型的肠细胞分化的特征。Caco-2细胞表达维生素A结合蛋白I和视黄醇结合蛋白II,角蛋白阳性。
    传代比例:1:2-1:4(Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱首次传代建议1:2)
    来源说明:Caco-2 Cells人结直肠腺癌细胞实验室复苏|STR图谱主要来源ATCC、ECACC、DSMZ、RCB等细胞库
    营养供给是细胞的生命基石。就像新生儿需要母乳或配方奶粉提供全面营养,初代细胞或刚刚复苏的冻存细胞对营养环境有着近乎苛刻的要求。科研人员会为这些""细胞婴儿""精心配制含有10-15%胎牛血清的基础培养基,这相当于为细胞准备的高级""配方奶粉""。血清中富含的生长因子、激素和附着因子,就像母乳中的免疫球蛋白和益生菌,能为细胞提供最贴心的保护。而对于某些娇贵的干细胞或免疫细胞,还需要额外添加EGF、bFGF等""营养强化剂"",这些细胞因子相当于婴幼儿的DHA和维生素补充剂,能显著提高细胞的贴壁率和增殖活性。实验室数据表明,在复苏后24小时内添加适量血清的细胞,其存活率可比常规培养提高30%以上。
    血清对温度极为敏感,必须严格控制在–20℃至–70℃的环境中保存。这一温度范围能够有效维持血清中各种生物活性成分的稳定性,包括生长因子、激素、蛋白质等。若因特殊情况需要将血清暂时存放于4℃冰箱,务必注意时间不得超过一个月,否则血清中的有效成分会逐渐降解,影响细胞培养效果。对于使用频率较低的实验室,建议将血清保存在–70℃超低温冰箱中,以最大限度延长其保质期。值得注意的是,即使在–20℃条件下,血清也不宜存放过久,通常建议在一年内使用完毕。
    HKb20 Cells人肾上皮细胞保种中心|STR图谱
    SK-MEL-1 Cells人皮肤黑色素瘤细胞保种中心|STR图谱
    B16-RFP Cells小鼠黑色素瘤细胞保种中心|STR图谱
    5637-Luc Cells人膀胱癌细胞保种中心|STR图谱
    Sf21 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:U-251_MG细胞、NHRF细胞、HT-3细胞
    Hs-343-T Cells;背景说明:详见相关文献介绍;传代方法:1:2—1:3传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:FL83B细胞、RCK-8细胞、HCT FET细胞
    CCK81 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HLE-B3细胞、IOSE29细胞、NCI H23细胞
    GM11889 Cells(提供STR鉴定图谱)
    HAP1 DIAPH1 (-) 2 Cells(提供STR鉴定图谱)
    B16-Luc Cells小鼠黑色素瘤细胞保种中心|STR图谱
    U-87MG-RFP Cells人脑星形胶质母细胞瘤细胞保种中心|STR图谱
    lewis-luc Cells小鼠肺癌细胞保种中心|STR图谱
    OCM-1A-RFP Cells人眼脉络黑色素瘤细胞保种中心|STR图谱
    CCD-841CoN Cells人正常结肠上皮细胞保种中心|STR图谱
    Anglne Cells人卵巢癌细胞保种中心|STR图谱
    WSU-DLCL(2) Cells;背景说明:弥漫大B淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:MDCK细胞、V 79-4细胞、HCV 29细胞
    H-4 Cells;背景说明:H4细胞系建系于1973年。它衍生于一个患神经胶质瘤的37岁病人的脑组织。该细胞的致瘤特性己经被屏蔽,细胞接种动物一般不产生肿瘤结节。该细胞具有修复MNNG损伤5型腺病毒的能力。;传代方法:1:3传代,每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MV522细胞、EOC20细胞、RBL 1细胞
    TE-3A Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:COLO 741细胞、GM06141B细胞、SuperTube细胞
    Leukemia L1210 Cells;背景说明:该细胞源于用0.2%甲基胆蒽(溶解)涂抹雌性小鼠的皮肤诱发的肿瘤,鼠痘病毒阴性。;传代方法:1:2传代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:Normal Rat, August 3, 1983细胞、NCI.H226细胞、HCGC细胞
    PTK2 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:JIII细胞、OVCAR3细胞、MDA-MB-435 S细胞
    FDC-P1 Cells;背景说明:详见相关文献介绍;传代方法:2-3天换液1次;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:CMT-93细胞、NCTC clone 929细胞、COLO 829细胞
    Medical University of Graz-Chordoma 1 Cells;背景说明:骶骨脊索瘤;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HEH2细胞、CA46细胞、B95-8细胞
    KU-19-19 Cells人膀胱癌细胞保种中心|STR图谱
    HEY Cells人卵巢癌细胞保种中心|STR图谱
    HGC-27+YFP Cells人胃癌细胞保种中心|STR图谱
    MUM2B Cells人侵袭性脉络膜黑色素瘤细胞保种中心|STR图谱
    JHH-2 Cells人肝癌细胞保种中心|STR图谱
    DU4475 Cells人乳腺上皮细胞保种中心|STR图谱
    MEL Cells小鼠红白血病细胞保种中心|STR图谱
    HGC27 Cells;背景说明:未分化胃癌,能分泌粘液素。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:SK BR 03细胞、MDA-134细胞、Hep G2细胞
    HHUUKDi007-A Cells(提供STR鉴定图谱)
    iOSE-C9 Cells(提供STR鉴定图谱)
    Ma-Mel-79 Cells(提供STR鉴定图谱)
    ND08030 Cells(提供STR鉴定图谱)
    SEWA/TC13 Cells(提供STR鉴定图谱)
    NIH/3T3-LUC Cells小鼠胚胎成纤维细胞保种中心|STR图谱
    PC-12-GFP Cells大鼠肾上腺嗜铬细胞瘤细胞保种中心|STR图谱
    PFIZi010-B Cells(提供STR鉴定图谱)
    Ubigene ID8 Kmt2c KO Cells(提供STR鉴定图谱)
    YAN3141 Cells(提供STR鉴定图谱)
    HG02809 Cells(提供STR鉴定图谱)
    HN 4 Cells;背景说明:喉鳞癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NTERA2细胞、EFM-192A细胞、NCI-H1915细胞
    MDBK Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:ECC10细胞、U-hth74细胞、HBZY1细胞
    251MG Cells;背景说明:U-251 MG分离至一位患者的胶质母细胞瘤组织。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:成纤维细胞样;相关产品有:Hs-578T细胞、IMCD-3细胞、HN 4细胞
    MV 3 Cells;背景说明:黑色素瘤;淋巴结转移;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MDAMB361细胞、UCLA SO M21细胞、Stanford University Pediatric T-cell line 1细胞
    OVCAR-3 Cells人卵巢癌细胞保种中心|STR图谱
    143B Cells人骨肉瘤细胞保种中心|STR图谱
    IM95m Cells人胃腺癌细胞保种中心|STR图谱
    BT483 Cells人乳腺导管癌细胞保种中心|STR图谱
    Panc 03.27 Cells人胰腺癌细胞保种中心|STR图谱
    NIH 3T3-RFP Cells小鼠胚胎成纤维细胞保种中心|STR图谱
    MC38 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:QG56细胞、H-1238细胞、OCM1A细胞
    Human fetal lung fibroblast 1 Cells;背景说明:详见相关文献介绍;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:成纤维细胞样;相关产品有:OCI AML3细胞、LC-1 sq细胞、WM239细胞
    S37 Cells;背景说明:肉瘤 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HBE135-E6E7细胞、Jurkat, Clone E6-1细胞、NCIH716细胞
    S37 Cells;背景说明:肉瘤 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HBE135-E6E7细胞、Jurkat, Clone E6-1细胞、NCIH716细胞
    S.B. Cells;背景说明:急性T淋巴细胞白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:BEL7402细胞、HEK-293-EBNA细胞、DU 145细胞
    SNU-620 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:DoHH2细胞、NPC-TW01细胞、NCIH1435细胞
    ACHN Cells人肾细胞腺癌细胞保种中心|STR图谱
    5637-Luc Cells人膀胱癌细胞保种中心|STR图谱
    MDS-L Cells人骨髓增生异常综合征细胞保种中心|STR图谱
    HCT 15 Cells人结直肠腺癌细胞保种中心|STR图谱
    Jurkat, Clone E6-1-Luc Cells人急性T淋巴细胞白血病细胞保种中心|STR图谱
    FTC-133 Cells人滤泡状甲状腺癌细胞保种中心|STR图谱
    GP5d Cells;背景说明:结肠癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:SKES-1细胞、OCI-AML2细胞、Vero细胞
    KM-12 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:T8细胞、MZCRC1细胞、U-251 MG细胞
    A375mel Cells;背景说明:A375源自一位54岁女性,是Giard DJ等人建立的一系列细胞株中的一株。该细胞可在免疫抑制小鼠上成瘤,在琼脂上形成克隆。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:上皮细胞样;相关产品有:SKLMS-1细胞、SGC-7901细胞、JHH2细胞
    KRC/Y Cells;背景说明:肾透明细胞癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:VMM5细胞、GT1-1细胞、P31/FUJ细胞
    S-16 Cells;背景说明:Schwann细胞;自发永生;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Hs578Bst细胞、Vero 76 clone E-6细胞、QGY 7701细胞
    SVEC4-10 Cells小鼠淋巴结内皮细胞保种中心|STR图谱
    NCI-H295R Cells人肾上腺皮质腺癌细胞保种中心|STR图谱
    HUVEC+RFP Cells人脐静脉内皮细胞保种中心|STR图谱
    NCI-H1650 Cells人非小细胞肺癌细胞保种中心|STR图谱
    DT40 Cells鸡淋巴瘤细胞保种中心|STR图谱
    NU-DUL-1 Cells人弥漫性组织细胞性淋巴瘤细胞保种中心|STR图谱
    MAC-T Cells牛乳腺上皮细胞保种中心|STR图谱
    Fao Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:上皮细胞;相关产品有:RPMI #8226细胞、Keio University-19-19细胞、BEL 7402细胞
    CL1.0 Cells;背景说明:肺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:COR-L 105细胞、M14-MEL细胞、MMAc-Serum Free细胞
    WI-38 Cells;背景说明:LeonardHayflick建系;有限传代细胞系;寿命为50±10代(倍增时间24h);来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人制备的人二倍体细胞;培养基中添加TNFα可以加快细胞生长。;传代方法:1:2-1:4传代;2-3天换液1次;生长特性:贴壁生长;形态特性:成纤维细胞样;相关产品有:RD细胞、LMTK-细胞、OCI-AML3细胞
    PANC403 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MFM223细胞、Hs294T细胞、H-929细胞
    PC-3M-IE8 Cells;背景说明:前列腺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MDA-MB-231-GFP细胞、M059J细胞、CCK-81细胞
    Hs 729T Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:MDA175细胞、3T3-F442A细胞、SW1463细胞
    WEHI231 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:LICR-HN6细胞、McA-RH 7777细胞、VMRC-RCZ细胞
    SUM-149 Cells;背景说明:乳腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:RAMOS2G64C10细胞、HMEC-1细胞、KYSE-150细胞
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    Next-generation characterization of the Cancer Cell Line Encyclopedia.
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    该产品被引用文献
    "PubMed=7459858
    Rousset M., Zweibaum A., Fogh J.
    Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
    Cancer Res. 41:1165-1170(1981)

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