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文献和实验The traditional method for combating RNases in enzymatic reactions such as in vitro transcription, reverse transcription and translation is to use human placental ribonuclease inhibitor (also known as RNase Inhibitor Protein, RI or hPRI
. No. 349523). The kit is composed of : CEN- chicken erythrocyte nuclei, used in setting cytometer's photomultiplier tube (PMT) voltages and amplifier gains, and providing information regarding instrument linearity and resolution; CTN - calf thymocyte
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
fragments containing EcoRI ends, use pBluescript II RI Predigested Vector (Stratagene). DNA gel stain (SYBR Safe, 10,000X concentrate in DMSO [dimethylsulfoxide]; Invitrogen) DNA ladder, 100-bp and/or 1-kb (New England Biolabs) DNA
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