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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
µl (15) Add 60 µl of Protein G agarose (50% slurry, preblocked, from EZ ChIP kit) into each chromatin sample. (16) Incubate for 1 hour at 4°C with horizontal rocking. This step removes non-specific binding activities
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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