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文献和实验Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
Adipose tissue-derived stem cells (ASCs) have been described for a number of laboratory animals and humans. Improved culture conditions and cellular characteristics of ASCs have been identified. ASCs can self-renew and differentiate
Isolation and Propagation of Glomerular Mesangial Cells
Cultures of glomerular mesangial cells (MC) of rodent or human origin have been extensively employed in renal research laboratories since the early 1980s. Cultured MC retain extensive analogies with the fairly undifferentiated in vivo phenotype
Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
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