Recombinant CD81/TAPA-1 Antibo

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

Intracellular Cytokine Staining Protocol

antibodies are also available in 20 µl/test sizes. If using these reagents, add 20 µl of pre-titrated antibody to the appropriate tube. Incubate in the dark at room temperature for 20 minutes. Add 1 ml of Permeabilization Buffer, centrifuge

Phage‐Based Expression Cloning to Identify Interacting Proteins

expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins