Monkey IL-1β (Interleukin 1 Be

Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1, IL-1, and Caspase-1

in primary murine bone marrow-�derived myeloid cells. The protocol encompasses cell handling, inflammasome activation and inhibition, as well as the detection of IL-1β, caspase-1, and IL-1α by ELISA and Western blot.

Measuring Inflammasome Activation in Response to Bacterial Infection

-1, a central mediator of innate immunity. The hallmarks of inflammasome activation are the processing of caspase-1, the maturation and release of interleukin-1β (IL-1β) and the induction of pyroptosis, a lytic inflammatory cell death. This protocol describes

浅谈流式核内蛋白染色步骤

就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定。为满足不同客户的实际需要和研究兴趣，BD于2005年初推出了CBA Flexset