Monkey TR (Targeted Ribonuclea

Microarray Analysis of DNA Replication Timing

is that it facilitates finer dissection of replication time in S-phase. Also, it helps delineate chromosomal regions that undergo biallelic or asynchronous replication, which otherwise are difficult to detect at a genome-wide scale by existing methods. The continuous TR

RNAi和基因沉默的历史回顾

). Recently, Ambion introduced a kit (the Silencer™ siRNA Construction Kit) to produce siRNAs by in vitro transcription, which is a less expensive alternative to chemical synthesis, particularly when multiple different siRNAs need to be synthesized

制备siRNAs的5种方法

合成法而言比较低，是一种性价比高的筛选siRNAs的好方法。更重要的是采用这种方法能够比化学合成法更快的得到siRNAs。以Silencer  siRNA Construction  Kit为例，一旦得到DNA Oligo模版（这个还是需要DNA合成的，不过DNA合成的成本就比较低了），只要24小时就可以，不需要等很久。这个方法的不足之处是实验的规模受到限制，虽然一次体外转录合成能提供足够做数百次转染的siRNAs，但是反应规模和量始终有一定的限制。而且和化学合成相比，还是需要占用研究人员相当的时间——毕竟