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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Filipin complex ready made solution
- 库存:
询盘
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
F755446-1ml
设定黄金标准,在阿拉丁,我们不仅相信保持质量,而且相信质量。 我们的目标是定义它。 我们的质量管理理念为业界研究试剂建立了高质量的标准。 当您听到阿拉丁这个名字时,就会知道它是各个方面坚定不移品质的灯塔。
中文名:Filipin complex 预制溶液
英文名:Filipin complex ready made solution
纯度:from Streptomyces filipinensis, 5mg/ml in DMSO based solution
SPU:F755446
CAS号:-
储存温度:-20°C储存
运输条件:超低温冰袋运输
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, gelatin or BSA to 100ug/ml, and/or non-ionic detergents such as Tween-20 or Nonidet P-40 or Triton X-100 (0.05 - 0.10% v/v) (Innis and Gelfand, 1990)。 Modern formulations may differ considerably, however - they are also generally proprietary. PCR
PCR PRIMER DESIGN AND REACTION OPTIMISATION
; however, it can lead to "non-specific" amplification and consequent reduction in yield of the desired product, if the 3'-most base is paired with a target. A consequence of too high a Ta is that too little product will be made , as the likelihood of primer
the annealing temperature correspondingly, you allow for more wobble (that is primer-template mismatch). A 50:50 ratio of AT to GC base pairs in the primer-template complex is optimal, but this may not always be obtainable. Primers with more GC than AT base
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