- ￥905.90
- 阿拉丁
- 上海
- B755514
- 2026年04月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存
- 英文名：
BCIP/NBT Liquid Substrate System
- 库存：
询盘
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
B755514-100ml
英文名：BCIP/NBT Liquid Substrate System
纯度或规格：ready to use solution
SPU：B755514
CAS：-
存储温度：2-8°C储存
运输条件：低温运输
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文献和实验Purification of acidic phosphatase from mustard seedlings
after 1 h by centrifugation in 50 ml centrifuge tube (approx. 35 ml per tube) at 31,000 x g ( estimate the rpm value using the rotor radius) for 30 min in the Hermle centrifuge at 4 °C (room B8220). 11. Decant the supernatant (SN2) into a grad
Purification of acidic phosphatase from mustard seedlings
and record the weight. (use 25-50 g). 2. Grind in a mortar with 75 ml of ice-cold water. 3. Make the volume up to 150 ml and transfer the slurry into the Polytron homogenizer. Homogenize 1 min at speed 5 (3x20 sec). 4. Filter
in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
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