- ￥2700
- AntibodySystem
- France
- VMC06701
- 2025年07月09日
- In vivo Functional Assay
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- 克隆性：
Monoclonal
- 适应物种：
Mouse
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 宿主：
Rat
- 应用范围：
In vivo Functional Assay
- 浓度：
1 mg/ml
- 靶点：
Csfgm, Csf2, Colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor, CSF, GM-CSF
- 抗体英文名：
InVivoMAb Anti-Mouse CSF2/GM-CSF Antibody (MP1-22E9)
- 抗体名：
InVivoMAb Anti-Mouse CSF2/GM-CSF Antibody (MP1-22E9)
- 规格：
100 μg
| 宿主 | Rat |
| 同种型 | IgG2a, kappa |
| 种属反应性 | Mouse |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 浓度 | 1 mg/ml |
| 纯度 | >95% as determined by SDS-PAGE. |
| 克隆性 | Monoclonal |
| 应用 | In vivo Functional Assay |
| 靶点 | Csfgm, Csf2, Colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor, CSF, GM-CSF |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 内毒水平 | < 1 EU/µg as determined by LAL test. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 克隆号 | MP1-22E9 |
| 备注 | For research use only. Not suitable for clinical or therapeutic use. |
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文献和实验Neutralizing Bioassay Protocols
describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In general, the cytokine bioassay protocols are modified to pre-incubate the cytokine of interest with the specific neutralizing antibody prior to addition
Intracellular Cytokine Staining Protocol
ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hr Monensin MP1-22E9 IFN-g mouse spleen ConA
Intracellular Immunofluorescent Staining for Flow Cytometry
GM-CSF mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A MP1-22E9 IFN
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