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文献和实验Long PCR Reagents and Guidelines
targets (1). 1X Long PCR Buffer 85 mM KOAc 25 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 8% glycerol 1% DMSO (1 to 4% works) 1.1-1.3 mM Mg(OAc)2 A 5X buffer stock containing 6 mM Mg(OAc)2 (1.2 mM final concentration
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Long-PCR Reagents and Guidelines
to 25 or 26 bases. We have not yet used this rule in our primer-picking but we are experimenting. REFERENCES 1. Cheng, S., Fockler, C., Barnes, W., Higuchi, R. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc
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