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文献和实验the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Materials 2.1— Construction of Adhesion Molecule—Ig Chimeras 2.2— Electroporation of Cells 2.3— Harvest and Culture of Transfectant Clones 2.4— Selection (ELISA) and Subcloning 2.5— Purification of Chimeric Proteins 3— Methods 3.1—
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