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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
实验试剂 1. CytoTune™ Sendai Reprogramming Vectors Note: For successful reprogramming, you need all four reprogramming vectors. 2. Human neonatal foreskin fibroblast cells to reprogram 3. Gibco® Mouse
to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
Activation and Expansion of Human Treg Cells
to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer
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