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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
PriCells: Isolation, culture, and purification of Human Pulmonary Microvascular Endothelial Cells
PriCells: Isolation, culture, and purification of Human Pulmonary Microvascular Endothelial Cells (HPMECs) 1. HPMECs were isolated from lungs resected from tissue of normal human or adult patients with malignant tumors. 2. Normal lung tissue
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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