Human GPR37 (G Protein Coupled

Visualization of G Protein−Coupled Receptor (GPCR) Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC)

of distinct oligomeric species is monitored with a ratiometric multicolor BiFC approach. Curr. Protoc. Neurosci. 51:5.29.1?5.29.15. © 2010 by John Wiley & Sons, Inc. Keywords: G protein?coupled receptor; oligomerization; bimolecular

Human DC Enrichment Kit

volume of 35 ml at room temperature. 2)        Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3)        Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4)        Remove 20 ml of supernatant

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

        g. Move the culture vessels in several quick back-and-forth and side-to-side motions to disperse the cells across the surface of the vessels.          h. Incubate the cells in a 37°C incubator with a humidified atmosphere of 5% CO2