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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20
- 保质期：
12个月
- 英文名：
Purified native IgM from 人serum, 100 ug 人重组蛋白
- 库存：
999
- 供应商：
上海圻明
- 规格：
50/100μg
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
(still on ice), and load immediately. For a gel thickness of 0.75mm and 15 wells applied 0.5 to 5ug for Coomasie Blue stain and 10 to 100-fold less protein for silver stain. If you do not know the electrophoretic pattern of your protein, load same sample
自然染色质免疫沉淀实验设计（Native Chromatin Immunoprecipitation (N-ChIP)）
has finished. 4. Immunoprecipitation 4.1.100-200ug unfixed chromatin + 100-200ul affinity purified antibody (50-100ug Ig) and the final volume made up to 1.0ml with incubation buffer. A negative control, with no added antibody, also needs to be set
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