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- WT-CB-N19378
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 保质期：
咨询
- 英文名：
Heparin disaccharide I-S, tetrasodium salt
- 库存：
100+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
136098-10-7
136098-10-7
分子式：C12H15NO19S3·4Na
分子量：665.4
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
onto a Pharmacia Hi-trap heparin column equilibrated in Buffer A.I found it easiest to load the sample onto the column by hand using a syringe and used the FPLC only for gradient salt elution. 3.Wash column with 5 column volumes of Buffer A by hand using a syringe
protein, Drosophila Wnt8 (Dwnt8 or WntD), indicating that this protocol is likelyapplicable to all Wnts. Key words: Wnt , Wnt3A , b-catenin , Purification , Blue Sepharose , Immobilized metal affinitychromatography (IMAC) , Gel filtration , Heparin
Growing feeder-independent embryonic stem cells§
before use. Trypsin solution: Add 100 mg of EDTA tetrasodium salt (Sigma) to 500 ml of PBS. Filter sterlize and add 10 ml (for 1×) or 20 ml (for 2×) of 2.5% trypsin solution (Gibco-BRL) and 5 ml of chicken serum (Gibco-BRL). Store in 20 ml aliquots
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