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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 保质期：
咨询
- 英文名：
Allyl b-D-lactose
- 库存：
100+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
52211-61-7
52211-61-7
分子式：C15H26O11
分子量：382.36
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文献和实验of RT Master Mix to each sample. 4. Incubate 42ºC, 2 hrs. B. RNA Hydrolysis 1. To each sample add: 12.5 1N NaOH 5 µL 0.5 M EDTA 2. Incubate at 65ºC, 15 min. 3. Neutralize each sample with: 20 µL 2M HEPES O
Use of the B.D. FACS or Calibur Flow Cytometers
Reservations: Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pay for both reserved time and the time actually used (which ever is greater). Prepare
Revised amino-allyl labeling protocol
for an additional 1 hour. B. Hydrolysis 1. Degrade RNA by addition of 15 m L of 0.1 N NaOH. Incubate at 70C for 10 minutes 2. Neutralize by addition of 15 m L 0.1 N HCl. To continue with the amino-allyl dye coupling procedure
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