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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984
Stewart Laboratory Standard Operating Procedure
+ 0.5% IFS (make fresh!). Incubate at RT for 20 minutes. Wash cells once with 1 mL wash buffer. Pellet and resuspend in 0.1 M sodium tetraborate (Na2B4O7). Incubate at RT for 2 minutes. Wash cells twice with 1 mL wash buffer (if a negative control of PI
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